Xiaojie Liu , Kai Chen , Zhongqi Wu

Institute of Life Sciences, Jiyang Colloge of Zhejiang A&F University, Zhuji, 311800, Zhejiang, China
Author    Correspondence author
Genomics and Applied Biology, 2024, Vol. 15, No. 2   doi: 10.5376/gab.2024.15.0013
Received: 27 Feb., 2024    Accepted: 04 Apr., 2024    Published: 18 Apr., 2024

This is an open access article published under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Liu X.J., Chen K., and Wu Z.Q., 2024, Advancements in gene editing technologies for mosquito research, Genomics and Applied Biology, 15(2): 107-119 (doi: 10.5376/gab.2024.15.0013)

The rapid advancements in gene editing technologies, particularly CRISPR/Cas9, have significantly impacted mosquito research, offering novel strategies for vector control and disease prevention. This study explores the latest developments in gene editing techniques applied to various mosquito species, including Anopheles, Aedes, and Culex. Key innovations such as the ReMOT Control method for efficient gene editing without embryo injections, the design and validation of guide RNAs, and the assessment of off-target effects in gene drives are discussed. Additionally, this study highlights the application of CRISPR/Cas9 in manipulating mosquito symbionts and developing gene drive systems to combat insecticide resistance. These advancements not only enhance our understanding of mosquito biology but also pave the way for sustainable and effective vector control strategies.

CRISPR/Cas9; Gene editing; Mosquito control; ReMOT control; Gene drive